"Tissue engineering approaches for tendon regeneration: using synergies between biological, biochemical and physical stimulus" by Prof. Manuela Gomes, University of Minho, Portugal
Date: 7 April 2016 Time: 11:00 - 12:00
Tendon tissue engineering (TE) requires tailoring scaffolds designs and properties to the anatomical and functional requirements of tendons located in different regions of the body. Cell sourcing is also of utmost importance as tendon cells are scarce. Recently, we have found that it is possible to direct the tenogenic differentiation of Amniotic fluid and Adipose tissue derived stem cells (hAFSCs and hASCs), and also that there are hASCs subpopulations that might be more prone to tenogenic differentiation. Nevertheless, biochemical stimulation may not be enough to develop functional TE substitutes for a tissue that is known to be highly dependent on mechanical loading.
These findings trigger our interest on in vitro biomechanically-stimulating culture environments that can be achieved modulating the scaffold architecture and composition and the stem cells. Particularly, the incorporation of magnetic nanoparticles (MNPs) within 3D constructs constitutes a novel and attractive strategy towards the development of magnetically-responsive system that may eventually combine therapeutic and diagnostic functionalities. An additional advantage is that cells naturally respond to magnetic forces, and consequently, the application of a magnetic field may enhance cell biological performance, and ultimately stimulate cell proliferation and differentiation. This work reports on recent studies concerning the development of specific scaffolds architectures based on various polymers, doped with MNPs and fabricated by either rapid prototyping technologies or electrospinning, enabling responsive systems for culturing stem cells, stimulating their tenogenic differentiation.
|Location:||SEMS Seminar room, Engineering Building, Mile End|