Close

A note on cookies

We use cookies to improve your experience of our website. If you want to find out more see our Privacy Policy

Menu

Institute of Bioengineering

People menu

Dr Thomas Iskratsch

Lecturer in Bioengineering

Department School of Engineering and Materials Science
 214, Engineering, Mile End
Telephone +44 (0)20 7882 3677
Email t.iskratsch@qmul.ac.uk
Homepage https://www.sems.qmul.ac.uk/staff/t.iskratsch

Research Keywords

Cardiomyocytes, Vascular Smooth Muscle Cells, Mechanosensing, Mechanosignalling, Rigidity Sensing, Myofibrillogenesis, Stem Cell Differentiation, Podosome Formation

Research Interests

Research Overview
The role of mechanical signals in the cardiovascular system

The cellular microenvironment is defined by both chemical and physical/mechanical parameters. Recent data inidicated that mechanical signals, such as extracellular matrix  rigidity contribute to guide cell migration, growth or differentiation during development or in disease.

Our research aims at understanding how mechanical forces regulate cell behavior especially in the cardiovascular system, including the formation and maintenance of the contractile myofibrils in cardiomyocytes during heart development or in cardiac disease, or the determination of vascular smooth muscle cell phenotypes (contractile or synthetic). To answer these questions we use techniques, such as nanopillar arrays, micro and nano-patterning,  magnetic tweezers or molecular Förster resonance energy transfer (FRET) protein activity and tension sensors.

Key Publications

Xanthis I and Iskratsch T (2019). Lamin-A Mechano-Protects the Heart. Developmental Cell  10.1016/j.devcel.2019.05.041

Pandey P, Hawkes W, Hu J, Megone WV, Gautrot J, Anilkumar N, Zhang M, Hirvonen L, Cox S, Ehler E, Hone J, Sheetz M and Iskratsch T (2018). Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions. Dev Cell  vol. 44, (3) 326-336.e3. 10.1016/j.devcel.2017.12.024

Tabdanov E, Gondarenko S, Kumari S, Liapis A, Dustin ML, Sheetz MP, Kam LC and Iskratsch T (2015). Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells. Integr Biol (Camb)  vol. 7, (10) 1272-1284. 10.1039/c5ib00032g

Iskratsch T, Wolfenson H and Sheetz MP (2014). Appreciating force and shape?the rise of mechanotransduction in cell biology. Nature Reviews. Molecular Cell Biology  vol. 15, (12) 825-833. 10.1038/nrm3903

Iskratsch T, Yu C-H, Mathur A, Liu S, Stévenin V, Dwyer J, Hone J, Ehler E and Sheetz M (2013). FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. Dev Cell  vol. 27, (5) 545-559. 10.1016/j.devcel.2013.11.003

2019

Sit B, Gutmann D and Iskratsch T (2019). Costameres, dense plaques and podosomes: the cell matrix adhesions in cardiovascular mechanosensing. J Muscle Res Cell Motil  10.1007/s10974-019-09529-7

Xanthis I and Iskratsch T (2019). Lamin-A Mechano-Protects the Heart. Developmental Cell  10.1016/j.devcel.2019.05.041

Pedrosa A-R, BODRUG N, Gomez-Escudero J, Carter EP, Reynolds LE, Georgiou PN, Fernandez I, Lees DM, Kostourou V, Alexopoulou AN, Batista S, Tavora B, Serrels B, Parsons M, Iskratsch T and Hodivala-Dilke K (2019). Tumour angiogenesis is differentially regulated by endothelial cell Focal Adhesion Kinase tyrosine-397 and -861 phosphorylation. American Association For Cancer Research  Cancer Research  10.1158/0008-5472.CAN-18-3934

Ward M and Iskratsch T (2019). Mix and (mis-)match - The mechanosensing machinery in the changing environment of the developing, healthy adult and diseased heart. Biochim Biophys Acta Mol Cell Res  10.1016/j.bbamcr.2019.01.017

2018

Ciuba K, Hawkes W, Tojkander S, Kogan K, Engel U, ISKRATSCH T and Lappalainen P (2018). Calponin-3 is critical for coordinated contractility of actin stress fibers. Nature Publishing Group  Scientific Reports  10.1038/s41598-018-35948-6

Pandey P, Hawkes W, Hu J, Megone WV, Gautrot J, Anilkumar N, Zhang M, Hirvonen L, Cox S, Ehler E, Hone J, Sheetz M and Iskratsch T (2018). Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions. Dev Cell  vol. 44, (3) 326-336.e3. 10.1016/j.devcel.2017.12.024

2017

Lee NM, Erisken C, Iskratsch T, Sheetz M, Levine WN and Lu HH (2017). Polymer fiber-based models of connective tissue repair and healing. Biomaterials  vol. 112, 303-312. 10.1016/j.biomaterials.2016.10.013

2016

Santos LC, Blair DA, Kumari S, Cammer M, Iskratsch T, Herbin O, Alexandropoulos K, Dustin ML and Sheetz MP (2016). Actin polymerization-dependent activation of Cas-L promotes immunological synapse stability. Immunol Cell Biol  vol. 94, (10) 981-993. 10.1038/icb.2016.61

Meacci G, Wolfenson H, Liu S, Stachowiak MR, Iskratsch T, Mathur A, Ghassemi S, Gauthier N, Tabdanov E, Lohner J, Gondarenko A, Chander AC, Roca-Cusachs P, O'Shaughnessy B, Hone J and Sheetz MP (2016). ?-Actinin links extracellular matrix rigidity-sensing contractile units with periodic cell-edge retractions. Mol Biol Cell  vol. 27, (22) 3471-3479. 10.1091/mbc.E16-02-0107

2015

Wolfenson H, Meacci G, Liu S, Stachowiak MR, Iskratsch T, Ghassemi S, Roca-Cusachs P, O'Shaughnessy B, Hone J and Sheetz MP (2015). Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft?matrices. Nat Cell Biol  vol. 18, (1) 33-42. 10.1038/ncb3277

Tabdanov E, Gondarenko S, Kumari S, Liapis A, Dustin ML, Sheetz MP, Kam LC and Iskratsch T (2015). Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells. Integr Biol (Camb)  vol. 7, (10) 1272-1284. 10.1039/c5ib00032g

2014

Wolfenson H, Iskratsch T and Sheetz MP (2014). Early events in cell spreading as a model for quantitative analysis of biomechanical events. Biophys J  vol. 107, (11) 2508-2514. 10.1016/j.bpj.2014.10.041

Iskratsch T, Wolfenson H and Sheetz MP (2014). Appreciating force and shape?the rise of mechanotransduction in cell biology. Nature Reviews. Molecular Cell Biology  vol. 15, (12) 825-833. 10.1038/nrm3903

Dwyer J, Pluess M, Iskratsch T, Dos Remedios CG and Ehler E (2014). The formin FHOD1 in cardiomyocytes. Anat Rec (Hoboken)  vol. 297, (9) 1560-1570. 10.1002/ar.22984

Zhang X, Moore SW, Iskratsch T and Sheetz MP (2014). N-WASP-directed actin polymerization activates Cas phosphorylation and lamellipodium spreading. J Cell Sci  vol. 127, (Pt 7) 1394-1405. 10.1242/jcs.134692

2013

Iskratsch T, Yu C-H, Mathur A, Liu S, Stévenin V, Dwyer J, Hone J, Ehler E and Sheetz M (2013). FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration. Dev Cell  vol. 27, (5) 545-559. 10.1016/j.devcel.2013.11.003

Iskratsch T, Reijntjes S, Dwyer J, Toselli P, Dégano IR, Dominguez I and Ehler E (2013). Two distinct phosphorylation events govern the function of muscle FHOD3. Cell Mol Life Sci  vol. 70, (5) 893-908. 10.1007/s00018-012-1154-7

Lynch CD, Lazar AM, Iskratsch T, Zhang X and Sheetz MP (2013). Endoplasmic spreading requires coalescence of vimentin intermediate filaments at force-bearing adhesions. Mol Biol Cell  vol. 24, (1) 21-30. 10.1091/mbc.E12-05-0377

2012

Roca-Cusachs P, Iskratsch T and Sheetz MP (2012). Finding the weakest link: exploring integrin-mediated mechanical molecular pathways. J Cell Sci  vol. 125, (Pt 13) 3025-3038. 10.1242/jcs.095794

Dwyer J, Iskratsch T and Ehler E (2012). Actin in striated muscle: recent insights into assembly and maintenance. Biophys Rev  vol. 4, (1) 17-25. 10.1007/s12551-011-0062-7

2011

Iskratsch T and Ehler E (2011). Formin-g muscle cytoarchitecture. Bioarchitecture  vol. 1, (2) 66-68. 10.4161/bioa.1.2.15467

2010

Iskratsch T, Lange S, Dwyer J, Kho AL, dos Remedios C and Ehler E (2010). Formin follows function: a muscle-specific isoform of FHOD3 is regulated by CK2 phosphorylation and promotes myofibril maintenance. J Cell Biol  vol. 191, (6) 1159-1172. 10.1083/jcb.201005060

2009

Herbst R, Iskratsch T, Unger E and Bittner RE (2009). Aberrant development of neuromuscular junctions in glycosylation-defective Large(myd) mice. Neuromuscul Disord  vol. 19, (5) 366-378. 10.1016/j.nmd.2009.02.011

Gutternigg M, Rendi¿ D, Voglauer R, Iskratsch T and Wilson IBH (2009). Mammalian cells contain a second nucleocytoplasmic hexosaminidase. Biochem J  vol. 419, (1) 83-90. 10.1042/BJ20081630

Iskratsch T, Braun A, Paschinger K and Wilson IBH (2009). Specificity analysis of lectins and antibodies using remodeled glycoproteins. Anal Biochem  vol. 386, (2) 133-146. 10.1016/j.ab.2008.12.005

^ Back to Top