Dr Tina ChowdhuryPhD
Senior Lecturer in Musculoskeletal Science
Our stimulating and vibrant team devoted to cross-disciplinary research provides an exciting environment focused on translational research particularly in the inflammatory joint disease domain. The aim of the mechanotherapy research group is to identify, characterise and clinically implement novel therapeutics that slow down inflammation and repair damage to soft tissues with the vision of restoring normal function. We use multi-disciplinary tools involving engineering/materials/biology and a variety of human tissues to investigate biomechanics and the pro-inflammatory mileau. Tissues include fetal membranes, cartilage and the intervertebral disc. Our research efforts are focused on translational investigation with the aim of delivering novel therapeutics that restore normal function and a healthy ageing phenotype into the clinical arena.
In the last two years, funding from Arthritis Research UK and the AO Foundation has led to substantial success in identifying a novel homeostatic pathway induced by C-type natriuretic peptide (CNP). This protein is found naturally occurring in cartilage tissue and activates natriuretic peptide receptor (Npr2) leading to cGMP/PrkgII signalling. Activation of this homeostatic pathway in response to CNP mediates both reparative and anti-inflammatory effects in diseased cartilage. Our work provides strong evidence that CNP based agents could be used to enhance repair and treat cartilage disorders such as osteoarthritis. In addition, the beneficial effects were enhanced with mechanical loading implicating that both signals (CNP and mechanical loading) act in an anti-inflammatory manner and have protective effects in joint tissues. The next step in the research is to replicate results in a diseased animal model before the benefits can be translated to patients. This is feasible because we are working closely with pharmacologist, Prof Adrian Hobbs (WHRI) and the clinical orthopaedic team led by Mr Manoj Ramachandran at the Royal London Hospital. This multi-disciplinary team makes the work clinically feasible in the next five years. Here is a short video of Dr Nick Peake (scientist funded by Arthritis Research UK) discussing our novel research on the protective effects of CNP in osteoarthritis.
Engels AC, Joyeux L, Van der Merwe J, Jimenez J, Prapanus S, Barrett DW, Connon C, Chowdhury TT, David AL and Deprest J (2017). Tissuepatch is biocompatible and seals iatrogenic membrane defects in a rabbit model. Prenat Diagn vol. 38, (2) 99-105. 10.1002/pd.5191
Barrett DW, Kethees A, Thrasivoulou C, Mata A, Virasami A, Sebire NJ, Engels AC, Deprest JA, Becker DL, David AL and Chowdhury TT (2017). Trauma induces overexpression of Cx43 in human fetal membrane defects. Prenatal Diagnosis vol. 37, (9) 899-906. 10.1002/pd.5104
Tilwani RK, Vessillier S, Pingguan-Murphy B, Lee DA, Bader DL and Chowdhury TT (2016). Oxygen tension modulates the effects of TNF? in compressed chondrocytes. Inflamm Res 10.1007/s00011-016-0991-5
Barrett DW, David AL, Thrasivoulou C, Mata A, Becker DL, Engels AC, Deprest JA and CHOWDHURY TT (2016). Connexin 43 is overexpressed in human fetal membrane defects after fetoscopic surgery. Prental Diagnosis 10.1002/pd.4917
Hossain MA, Chowdhury T and Bagul A (2015). Imaging modalities for the in vivo surveillance of mesenchymal stromal cells. J Tissue Eng Regen Med vol. 9, (11) 1217-1224. 10.1002/term.1907
Peake NJ, Bader DL, Vessillier S, Ramachandran M, Salter DM, Hobbs AJ and Chowdhury TT (2015). C-type natriuretic peptide signalling drives homeostatic effects in human chondrocytes. Biochem Biophys Res Commun vol. 465, (4) 784-789. 10.1016/j.bbrc.2015.08.087
Peake NJ, Pavlov AM, D'Souza A, Pingguan-Murphy B, Sukhorukov GB, Hobbs AJ and CHOWDHURY TT (2015). Controlled Release of C-Type Natriuretic Peptide by Microencapsulation Dampens Proinflammatory Effects Induced by IL-1? in Cartilage Explants. American Chemical Society Biomacromolecules 10.1021/bm501575w
Chowdhury B, David AL, Thrasivoulou C, Becker DL, Bader DL and Chowdhury TT (2014). Tensile strain increased COX-2 expression and PGE2 release leading to weakening of the human amniotic membrane. Placenta vol. 35, (12) 1057-1064. 10.1016/j.placenta.2014.09.006
Peake NJ, Hobbs AJ, Pingguan-Murphy B, Salter DM, Berenbaum F and Chowdhury TT (2014). Role of C-type natriuretic peptide signalling in maintaining cartilage and bone function. Osteoarthritis Cartilage vol. 22, (11) 1800-1807. 10.1016/j.joca.2014.07.018
Peake NJ, Hobbs AJ, Pingguan-Murphy B, Salter DM, Berenbaum F and CHOWDHURY TT (2014). Role of C-type natriuretic peptide signaling in maintaining cartilage and bone function. Elsevier/Science Direct Osteoarthritis and Cartilage vol. online edition, 10.1016/j.joca.2014.07.018
Hossain MA, CHOWDHURY TT and Bagul A (2014). Imaging modalities for the in vivo surveillance of mesenchymal stromal cells. Wiley Journal of Tissue Engineering and Regenerative Medicine 10.1002/term.1907
Parker E, Vessillier S, Pinguaan-Murphy B, Wan Abas WAB, Bader DL and Chowdhury TT (2013). Low oxygen tension increased FN-f induced catabolic activities - response prevented with biomechanical signals. Biomed Central Arthritis Research and Therapy vol. 15, (5) R163-R163. 10.1186/ar4346
Peake N, Su N, Ramachandran M, Achan P, Salter DM, Bader DL, Moyes AJ, Hobbs AJ and Chowdhury TT (2013). Natriuretic peptide receptors regulate cytoprotective effects in a human ex vivo 3D/bioreactor model. Arthritis Research and Therapy vol. 15, (4) 10.1186/ar4253
Akanji O, Salter DM and Chowdhury TT (2013). Optimisation of quantitative real-time PCR studies in cartilage mechanobiology. Pcr Technology. Current Innovations , Editors: Nolan T and Bustin SA. Crc Pressi Llc
Hossain MA, Frampton AE, Chowdhury TT and Morsey M (2013). Biological vascular grafts for hemodialysis access. Informa Healthcare Expert Rev Med Devices vol. 2, (10) 171-175. 10.1586/erd.12.85
CHOWDHURY TT, Tilwani RK and Bader DL (2012). Biomechanical conditioning enhanced matrix synthesis in nucleus pulposus cells cultured in agarase constructs with TGF?, Editors: Goldsmith EC and Lin SK. Journal Functional Biomaterials vol. 3, 23-26. 10.3390/jfb3010023
Ramachandran M, Achan P, Salter DM, Bader DL and Chowdhury TT (2011). Biomechanical signals and the C-type natriuretic peptide counteract catabolic activities induced by IL-1? in chondrocyte/agarose constructs. Arthritis Research and Therapy vol. 13, (5) 10.1186/ar3459
Lee DA, Brand J, Salter D, Akanji O-O and Chowdhury TT (2011). Quantification of mRNA using real-time PCR and Western blot analysis of MAPK events in chondrocyte/agarose constructs. Methods Mol Biol vol. 695, 77-97. 10.1007/978-1-60761-984-0_6
Bader DL, Salter DM and Chowdhury TT (2011). Biomechanical influence of cartilage homeostasis in health and disease. Arthritis vol. 2011, 10.1155/2011/979032
Chowdhury TT, Schulz RM, Rai SS, Thuemmler CB, Wuestneck N, Bader A and Homandberg GA (2010). Biomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte/agarose constructs. Arthritis Research and Therapy vol. 12, (3) 10.1186/ar3009
Akanji OO, Sakthithasan P, Salter DM and Chowdhury TT (2010). Dynamic compression alters NF?B activation and I?B-? expression in IL-1?-stimulated chondrocyte/agarose constructs. Inflammation Research vol. 59, (1) 41-52. 10.1007/s00011-009-0068-9
McGlashan SR, Knight MM, Chowdhury TT, Joshi P, Jensen CG, Kennedy S and Poole CA (2010). Mechanical loading modulates chondrocyte primary cilia incidence and length. Cell Biology International vol. 34, (5) 441-446. 10.1042/CBI20090094
Raveenthiran SP and Chowdhury TT (2009). Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Biomechanics and Modeling in Mechanobiology vol. 8, (4) 273-283. 10.1007/s10237-008-0134-1
Chowdhury TT, Salter DM, Bader DL and Lee DA (2008). Signal transduction pathways involving p38 MAPK, JNK, NF kappa B and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1 beta and dynamic compression. Inflamm Res vol. 57, (7) 306-313. 10.1007/s00011-007-7126-y
Chowdhury TT, Arghandawi S, Brand J, Akanji OO, Bader DL, Salter DM and Lee DA (2008). Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. Arthritis Res Ther vol. 10, (2) 10.1186/ar2389
CHOWDHURY TT, Akanjii OO, Salter DM and Lee DA (2008). Dynamic compression influences IL-1? induced NO and PGE2 release, via alterations in iNOS and COX-2 activity. 5th International Symposium On Mechanobiology of Cartilage and Chondrocyte , Editors: Stoltz JF.
Chowdhury TT, Akanji OO, Salter DM, Bader DL and Lee DA (2008). Dynamic compression influences interleukin-1 beta-induced nitric oxide and prostaglandin E-2 release by articular chondrocytes via alterations in iNOS and COX-2 expression. Biorheology vol. 45, (3-4) 257-274. 10.3233/BIR-2008-0474
Campbell JJ, Blain EJ, Chowdhury TT and Knight MM (2007). Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes. Biochemical and Biophysical Research Communications vol. 361, (2) 329-334. 10.1016/j.bbrc.2007.06.185
Chowdhury TT and Knight MM (2006). Purinergic pathway suppresses the release of NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compression. Journal of Cellular Physiology vol. 209, (3) 845-853. 10.1002/jcp.20768
Chowdhury TT, Appleby RN, Salter DM, Bader DA and Lee DA (2006). Integrin-mediated mechanotransduction in IL-1 beta stimulated chondrocytes. Biomech Model Mechanobiol vol. 5, (2-3) 192-201. 10.1007/s10237-006-0032-3
Chowdhury TT, Bader DL and Lee DA (2006). Anti-inflammatory effects of IL-4 and dynamic compression in IL-1beta stimulated chondrocytes. Biochem Biophys Res Commun vol. 339, (1) 241-247. 10.1016/j.bbrc.2005.11.016
Chowdhury TT, Bader DL and Lee DA (2006). Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Biorheology vol. 43, (3,4) 413-429.
CHOWDHURY TT, Bader DL and Lee DA (2006). Dynamic compression counteracts IL-1? induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Mechanobiology: Cartilage and Chondrocyte , Editors: Stoltz JF. Ios Press
Chowdhury TT, Appleby RN, Salter DA, Bader DL and Lee DA (2005). Integrin mediated mechanotransduction in IL-1 stimulated bovine chondrocytes cultured in agarose constructs. Proceedings of The 2005 Summer Bioengineering Conference vol. 2005, 545-546.
Chowdhury TT, Bader DL and Lee DA (2005). Dynamic compression counteracts IL-1-induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Proceedings of The 2005 Summer Bioengineering Conference vol. 2005, 251-252.
Catuogno C, Chowdhury TT, Bader DL and Lee DA (2005). Dynamic compression influences the biochemical response of human mesenchymal stem cells cultured in agarose constructs. European Cells and Materials vol. 10, (SUPPL.2)
Chowdhury TT, Bader DL and Lee DA (2005). Dynamic compression counteracts IL-1? induced INOS and COX-2 activity by human chondrocytes cultured in agarose constructs. European Cells and Materials vol. 10, (SUPPL.2)
Sudre L, Cheung F, Kevorkian L, Young DA, Darrah C, Donell ST, Shepstone L, Porter S, Brockbank S, Edwards DR, Parker AE, Clark IM, Boubriak OA, Urban JPG, Cui Z, Tew SR, Li Y, Tweats LM, Hawkins RE, Hardingham TE, Green D, Partridge KA, Leveque I, Mann S, Oreffo ROC, Ball SG and Kie (2005). British society for matrix biology autumn meeting. International Journal of Experimental Pathology vol. 86, (3) 10.1111/j.0959-9673.2005.00426.x
Chowdhury TT, Salter DM, Bader DL and Lee DA (2004). Integrin-mediated mechanotransduction processes in TGFbeta-stimulated monolayer-expanded chondrocytes. Biochem Biophys Res Commun vol. 318, (4) 873-881. 10.1016/j.bbrc.2004.04.107
Bryant SJ, Chowdhury TT, Lee DA, Bader DL and Anseth KS (2004). Crosslinking density influences chondrocyte metabolism in dynamically loaded photocrosslinked poly(ethylene glycol) hydrogels. Ann Biomed Eng vol. 32, (3) 407-417. 10.1023/B:ABME.0000017535.00602.ca
Chowdhury TT, Bader DL and Lee DA (2003). Dynamic compression counteracts IL-1 beta-induced release of nitric oxide and PGE2 by superficial zone chondrocytes cultured in agarose constructs. Osteoarthritis Cartilage vol. 11, (9) 688-696. 10.1016/S1063-4584(03)00149-3
Chowdhury TT, Bader DL, Shelton JC and Lee DA (2003). Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression. Arch Biochem Biophys vol. 417, (1) 105-111. 10.1016/S0003-9861(03)00340-0
Chowdhury TT, Shelton JC, Bader DL and LEE DA (2003). Control of mechanotrasduction processes in dynamically stimulated human and bovine chondrocytes cultured in agarose constructs. Internat. J. Artificial Organs vol. 26, (9) 858-858.
Chowdhury TT, LEE DA, Salter DM, Shelton JC and Bader DL (2003). alpha5beta1 integrin mediates mechanotransduction processes in TGFbeta-stimulated human chondrocyte / agarose constructs and subjected to dynamic compression. Osteoarthritis Cartilage vol. 11, (Suppl 1) S2-S3.
Chowdhury TT, Bader DL and Lee DA (2001). Dynamic compression inhibits the synthesis of nitric oxide and PGE(2) by IL-1beta-stimulated chondrocytes cultured in agarose constructs. Biochem Biophys Res Commun vol. 285, (5) 1168-1174. 10.1006/bbrc.2001.5311